Establish minipig model of type 2 diabetes mellitus with high-fat and high-sucrose diet

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Objective To establish minipig model of type 2 diabetes mellitus with high-fat and high-sucrose diet and to observe the changes of serum glucagon-like peptide-1(GLP-1) and its relation with blood glucose, insulin and C-peptide. Methods Twenty 4-month-old Chinese experimental minipigs(weighted (16.9±1.1)kg) were randomly assigned to the control diet group (CD group, n=10) and high-fat and high-sucrose diet (HFSD group, n=10), with 5 females and 5 males in each group, according to the random number table. CD group were fed with basic diet, and HFSD group were fed with high-fat and high-sucrose diet to establish type 2 diabetic minipig model. Fasting blood glucose≥7.0 mmol/L was set as the criterion that the type 2 diabetic minipig model were established successfully. Blood samples for plasma parameters, including blood glucose, insulin, C-peptide and blood lipid, were withdrawn from the animals separately at the end of the 0, 3rd and 6th month following an overnight fasting and 2 hours after feeding, and for detecting the blood GLP-1. The t test was performed to compare the data of the two groups. Pearson's correlation analysis was used to investigate the relationship between GLP-1 and the other variables. Results At the end of the 6th month, the average weight of HFSD group was (74±11) kg and that of CD group was (44±4) kg (t=7.93, P 0.01). Conclusions A 6-month of feeding with high-fat and high-sucrose diet can establish an ideal type 2 diabetes model in minipig, which features weight gain, blood glucose elevation, increased levels of serum TG and TC, and significantly decreased GLP-1 level. Key words: Diabetes mellitus, type 2; Models, animal; Chinese experimental minipigs; Glucagon-like peptide-1