Establish and optimize the model of experimental allergic encephalomyelitis in mice

Abstract

Objective To explore the appropriate dosage of drugs inducing experimental allergic encephalomyelitis (EAE) in mice, and evaluate the modified model mice. Methods Different doses of myelin oligodendrocyte glycoprotein (MOG35-55: 200 μg, 100 μg, 50 μg, 25 μg), together with different doses of inactivation of mycobacterium tuberculosis (H37RA: 800 μg, 250 μg, 100 μg) and pertussis toxin(500 ng, 200 ng), were used to induce the EAE model. After immunized, the clinical disease severity of EAE mice was measured by the standard EAE grading clinical score daily. The open field test was used to detect the locomotion of mice. The Western blot and immunofluorescence were used to detect the level of myelin basic proteins (MBP) in different brain regions of mice. Results Compared with the EAE mice induced with high-dose drugs, the mice with low-dose drugs (25 μg MOG35-55, 100 μg H37RA, 200 ng pertussis toxin) had low neurological scores. And they displayed normal locomotion compared with the control mice (day 16: group EAE(8.885±0.772)cm/s vs control group (8.933±0.567)cm/s, P>0.05; day 31: group EAE (11.130±0.630)cm/s vs control group (10.670±0.959)cm/s, P>0.05; day 55: group EAE (7.686±0.428)cm/s vs control group (8.313±0.918)cm/s, P>0.05). Moreover, there was a significant decrease of MBP in the parahippocampal cortex (PHC) and fimbria-fornix of EAE mice induced with low-dose of drugs (PHC: group EAE (0.369±0.096) vs control group (1.000±0.163), P<0.05; fimbria-fornix: group EAE (0.494±0.071) vs control group (1.000±0.143), P<0.05). Conclusion The EAE mice induced with low-dose drugs(25 μg MOG35-55, 100 μg H37RA, 200 ng pertussis toxin) have low neurological scores, normal locomotion, and myelin impairment in the central neuronal system. And it can be used in the cognitive behavioral research of demyelination disease, such as multiple sclerosis. Key words: Experimental allergic encephalomyelitis (EAE); Neurological score; Locomotion; Myelin impairment