Establecimiento de una línea celular primaria apartir de huevos con embrión de Toxocara canis

Abstract

IntroductionToxocara canis is the second most prevalent nemathelminthes in dogs at regional level and among the three most frequent in some countries in the region. Due to the fact that the dog is the contamination source, it becomes a nematode with a high zoonotic potential, so we consider it important to be able to use the cell line of this helminth to study the basic aspects, as well as the development of diagnostic techniques. ObjectiveTo obtain a primary cell line from embryonated eggs of T.canis. MethodsThe parasites were extracted from the small intestines of dogs under one year old. Embryonic cells were obtained by embryogenesis of the eggs secreted by adult worms in four different media; two were rich in nutrients, one was 1% formaldehyde, and the other was distilled water. The cells were obtained by mechanical dissociation of embryonated eggs using 30G needles. ResultsThe estimated time for obtaining the cell line was fifteen days, from which seven were used for egg embryogenesis. The cells responded positively to the cryopreservation methods after two days or even two months, allowing a replication phase with four passes. ConclusionsWe managed to obtain a cell line from T. canis embryonated eggs. This cell line will help the understanding of pathogenic relationships, potential therapeutic targets and for developing diagnostic methods.