Abstract
The fast advancement and deployment of sequencing technologies after the Human Genome Project have greatly increased our knowledge of the eukaryotic genome sequences. However, due to technological concerns, high-quality genomic data has been confined to a few key organisms. Moreover, our understanding of which portions of genomes make up genes and which transcript isoforms synthesize these genes is scarce. Therefore, the current study has been designed to explore the reliability of the tiger lily (Lilium lancifolium Thunb) transcriptome. The PacBio-SMRT was used for attaining the complete transcriptomic profile. We obtained a total of 815,624 CCS (Circular Consensus Sequence) reads with an average length of 1295 bp. The tiger lily transcriptome has been sequenced for the first time using third-generation long-read technology. Furthermore, unigenes (38,707), lncRNAs (6852), and TF members (768) were determined based on the transcriptome data, followed by evaluating SSRs (3319). It has also been revealed that 105 out of 128 primer pairs effectively amplified PCR products. Around 15,608 transcripts were allocated to 25 distinct KOG Clusters, and 10,706 unigenes were grouped into 52 functional categories in the annotated transcripts. Until now, no tiger lily lncRNAs have been discovered. Results of this study may serve as an extensive set of reference transcripts and help us learn more about the transcriptomes of tiger lilies and pave the path for further research.
Highlights
The tiger lily (Lilium lancifolium Thunb) grows from bulbs producing corolla-shaped flowers of various colors, such as orange, red, yellow, and white [1]
The full genomic and transcriptomic data would be extremely useful for studying the gene models of the tiger lily
The RNA obtained from 5 plant samples was combined and sequenced on a Pacific Biosciences (PacBio) Sequel system in order to acquire full-length transcriptomes (FLTs) data
Summary
The tiger lily (Lilium lancifolium Thunb) grows from bulbs producing corolla-shaped flowers of various colors, such as orange, red, yellow, and white [1]. The tiger lily is wellknown across the globe for its beautiful look as well as is used as a nutritious food. The Chinese Ministry of Health’s medical plants listed tiger lily as a medicinal plant because of its abundant minerals and bioactive constituents including alkaloids, phenolic glycosides, pectin, and vitamins. The tiger lily has a large genome, ranging from 32.8 to 47.9 pg [2], as a triploid plant. The full genomic and transcriptomic data would be extremely useful for studying the gene models of the tiger lily. The enormous and unidentified genome, as well as the high heterozygosity, makes the functional evaluation of genes challenging
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