Abstract
This study aimed to investigate the effects of inoculation with the arbuscular mycorrhizal fungus (AMF) Rhizophagus clarus treatment with humic substances, and phosphorus (P) fertilization on the essential oil content and composition of Lippia alba (Mill.) N.E.Br. The experiment was conducted in a completely randomized design with a 2 × 2 × 2 factorial arrangement (AMF, P, and humic substances). Essential oil content was higher in AMF-inoculated plants treated with a high P rate. A total of 42 compounds were identified in the essential oil, and the predominant classes were oxygenated monoterpenes and sesquiterpene hydrocarbons. The major compounds were carvone (28.85–44.37%), (E)-caryophyllene, and limonene. Given its essential oil composition, L. alba was classified as of the carvone–limonene chemotype. AMF + P + humic substances treatment led to a decrease in carvone content compared with humic substances treatment. It was concluded that AMF inoculation combined with a high P rate increased essential oil content, whereas AMF inoculation combined with a high P rate and humic substances addition decreased carvone content.
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