Abstract
Total phenols, flavonoids, flavonols, and flavanols of the methanolic extract of the aerial part of Artemisia herba-alba were determined. The extract was analyzed by liquid chromatography with photodiode array coupled with electrospray ionisation mass spectrometry and allowed to identify of 10 phenolic compounds. Apigenin-6-C-glycosyl flavonoids and caffeoylquinic acids were identified. Chlorogenic acid and 1,4 dicaffeoylquinic acid being the major constituents. The essential oil obtained by hydrodistillation was analyzed by gas chromatography–mass spectrometry. Twenty-three compounds, representing 97.8% of the total oil, were identified. The most abundant components were β-thujone (41.9%), α-thujone (18.4%), and camphor (13.2%). Methanolic extract and essential oil exhibited a considerable antioxidant activity as evaluated by 2,2-diphenyl-pycrilhydrazil hydrate scavenging activity, reducing power, β-carotene bleaching test, and chelating ability. The methanolic extract was found to be more efficient, while the essential oil exhibited the highest acetylcholinesterase inhibitory activity. Analysis of the antibacterial activity showed that A. herba-alba methanolic extract and essential oil are efficient against gram positive and gram negative bacteria.
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