Abstract
This study aims to investigate the resistance of E. coli O157:H7 to acetic acid (AA) or malic acid (MA) by adding osmolytes, such as glycerol, glycine, glucose, and fructose, in Luria-Bertani broth without NaCl (LBW/S) or phosphate buffer (PB) stored at 25 °C. In LBW/S, a significantly (p < 0.05) higher D-value of E. coli O157:H7 was observed when treated with AA and 20% glycine (D-value: 1.18–3.44) or 40% glucose (D-value: 1.05–2.52) compared to that of AA alone (D-value: 0.40–0.47). In contrast, the addition of osmolytes (i.e. 3-40% glucose, 3–40% fructose or 20% glycine) to LBW/S acidified by MA significantly decreased D-values of E. coli O157:H7, which was enumerated by using a selective medium. Furthermore, when E. coli O157:H7 was incubated in LBW/S containing AA and osmolytes at 25 °C for 3 d, this bacterium had an increased proportion of C16:0 and C17:0 cyclo (cyclopropane acid) compared to its AA-treated counterparts. Along with the altered shift in membrane phospholipids, the addition of osmolytes into a laboratory medium in the presence of nutritive substrates may increase the resistance of E. coli O157:H7 to AA.
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