Escherichia coli lipopolysaccharide modulates bovine luteal cell function

E Grant,I M Sheldon,S Herath,S T Lilly

doi:10.1136/vr.161.20.695

Abstract

<i>Escherichia coli</i> lipopolysaccharide modulates bovine luteal cell function

Highlights

An important cause of subfertility in cattle is prolonged luteal phases of the oestrous cycle associated with postpartum metritis or endometritis (Opsomer and others, 2000)
The stage of the oestrous cycle was determined using the criteria of Ireland and others (1980) and only corpora lutea from the mid-luteal phase were used in the present study
To test if the luteal cells were functional in vitro, they were treated with luteinising hormone (LH); control cultures were supplied with media

Summary

Introduction

An important cause of subfertility in cattle is prolonged luteal phases of the oestrous cycle associated with postpartum metritis or endometritis (Opsomer and others, 2000). The stage of the oestrous cycle was determined using the criteria of Ireland and others (1980) and only corpora lutea from the mid-luteal phase were used in the present study. The method of luteal cell isolation and culture was as previously described (Pate and Condon 1982).

Results

Conclusion