Escherichia coli Clonal Variability Based on Genetic Diversity Pattern with Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction Methods for Traveler’s Diarrhea Cases in Bali

Abstract

BACKGROUND: Bali is a favorite tourism destination in the world. As a major tourist destination, the incidence of illness that afflicts tourists greatly affects the image of tourism. Diarrhea is a health problem that is most often experienced and is a major obstacle for foreign tourists when traveling, especially to Bali. Escherichia coli (E. coli) bacteria cause diarrhea more often than viruses in some developing countries. Genetic differences can affect the characteristics of E. coli, especially in relation to the medical field. AIM: We would like to assess the genetic diversity of the different pathogenic E. coli from various clinical isolates including those from traveler’s diarrhea in Bali, Indonesia. MATERIALS AND METHODS: One of the molecular techniques used in this study is to use enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). The sample in this study was the feces of foreign tourists with traveler’s diarrhea in Bali. This study carried out research procedures in the form of Isolation of E. coli genome DNA from culture, amplification of E. coli 16S rRNA encoding genes, sequencing of E. coli 16S rRNA encoding genes, phylogenetic tree construction, and then analysis of E. coli genetic diversity with ERIC-PCR sequences. RESULTS: The results showed that the ERIC-PCR method was more discriminatory than other methods to analyze the genetic diversity of E. coli from fecal samples of patients with traveler’s diarrhea. It was found that clonal variability based on the genetic similarity of all sample E. coli isolates varied from 0% to 100%. CONCLUSIONS: This shows that the source of transmission and the strains of E. coli that cause it comes from diverse populations.