Escherichia coli and phosphate interplay mediates transport of nanoscale zero-valent iron synthesized by green tea in water-saturated porous media

Abstract

Green synthesized nano-zero-valent iron (GT-nZVI) has been considered an excellent material for in-situ soil remediation due to its high stability and environmental benignity. However, sufficient transportability of GT-nZVI downstream towards the contaminated sites, likely affected by the physicochemical properties of soil-groundwater, is required for improved in-situ remediation. Thus, the effect of soil components (i.e., bacteria and phosphate) on GT-nZVI transportability is significant. Hence, we studied the transport of GT-nZVI (Fe0 core wrapped by green tea polyphenols) with the existence of E. coli and phosphate in water-saturated porous sand media in NaCl and CaCl2 solutions at pHs 6.0 and 8.0. Also studied were the stability, surface characteristics, and two-site kinetics attachment modeling (TKAM) with Escherichia coli or/and phosphate. The results showed that phosphate could further enhance GT-nZVI co-transport with E. coli by increasing the negative charge on GT-nZVI at pH 6.0. However, E. coli reduced GT-nZVI mobility at pH 8.0 because the cell-cell interactions could mask the negative charges of pre-deposited GT-nZVI on E. coli, forming the large clusters between GT-nZVI and E. coli. Then, phosphate occurrence diminished E. coli inhibition by detaching GT-nZVI from nZVI-E. coli-phosphate polymers due to the stronger phosphate adsorption on E. coli than GT-nZVI at pH 8.0. Overall, TKAM describes the transport and retention of GT-nZVI adequately under various conditions, indicating the deposition order with k2str value as follows: GT-nZVI alone > with (w.) E. coli > w. phosphate > w. combined E. coli & phosphate at pH 6.0. By contrast, w. phosphate > w. E. coli > w. combined E. coli & phosphate > GT-nZVI alone ensued at pH 8.0. This investigation highlights the transport behavior of GT-nZVI associated with surface property changes in complex environments for effective in-situ remediation.