Abstract
In the paper by Qin et al. (2014), there were errors in Figure 1. The correct figure is reproduced here. Effects of silencing endothelial nitric oxide synthase (eNOS) gene with shRNA plasmid (A) H9c2 cardiomyocytes were successfully transfected with shRNA plasmid of eNOS, which presented with green fluorescence (200x, fluorescence microscope). (B) Comparison of mtDNA copy number between the cells treated only with 20 U/mL recombinant human erythropoietin (rhEPO) (EPO control) and those treated with 20 U/mL rhEPO and shRNA plamid (EPOþshRNA), based on the cytochrome c/b-actin ratio. (C) Comparison of peroxisome profilerator activated receptor gamma coactivator alpha (PGC-1a) mRNA expression, normalized by ribosomal protein L10A (RPL10A) mRNA. (D) Comparison of the expression and phosphorylation of endothelial nitric oxide synthase (eNOS). p-eNOS: phosphorylated eNOS. #P < 0.05 versus cells treated only with 20 U/mL rhEPO.
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