Abstract
Morphological and functional evidence exists that macrophages support in vivo erythropoiesis. Using in vitro cultured primary adherent cells from murine bone marrow as antigenic source, we have prepared a hybridoma cell line secreting a monoclonal antibody (mAb) to reticular cells of bone marrow and non-lymphoid domains in the spleen. The mAb (α-ER-HR3) also binds to some elongated cells in the subcutaneous tissue and to intertubular areas of the renal medulla. In addition, the ER-HR3 antigen is expressed on some reticular cells in the capsular sinuses and paracortex of lymph nodes and in the lamina propria of the ilium and colon, with increasing expression towards the distal ileum. The expression of the ER-HR3 antigen in non-lymphoid domains of hemopoietic organs is proposed to be associated with adult type hemoglobin (Hb) erythropoiesis as evidenced by (1) the absence in the yolk sac, (2) the exact correlation with the presence of adult type Hb erythropoiesis, but not granulopoiesis, in fetal and neonatal liver and spleen and postnatal bone marrow and spleen, and (3) the correlation with phenylhydrazine-induced hepatic erythropoiesis in the adult liver.
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