Erythromycin residue in honey from the Southern Marmara region of Turkey

Abstract

Honey samples, collected from the Southern Marmara region of Turkey, were analysed for erythromycin residues by liquid chromatography–mass spectrometry using electrospray ionization in the positive ion mode (LC–ESI–MS). Fifty samples, comprising chestnut, pine, linden and multi-flower honeys, were collected directly from hives and analyzed. The limit of detection and quantification were 6 and 20 ng g−1, respectively, and recovery ranged from 85 to 89%. Four of the honey samples (8%) were found to be contaminated with erythromycin residues at concentrations ranging from 50 to 1776 ng g−1. An erythromycin-fortified cake feeding assay was also performed in a defined hive to test the transfer of erythromycin residue to the honey matrix. In this test hive, the residue level in the honey, 3 months after dosing, was approximately 28 ng g−1.