Abstract
Summary. A simple suspension type culture system has been described for the in vitro maintenance of erythroid cells obtained from adult human bone marrow. In the presence of erythropoietin, normoblasts of all stages of maturation were still present after more than 10 days in culture. Following an initial decline, the numbers of basophilic normoblasts showed an absolute increase, reaching a maximum after about 5 days. Evidence of continued DNA synthesis in these cells, the observation of mitotic figures and the tracing of [3H]thymidine label from early normoblasts to intermediate and late normoblasts in culture further suggests that these cells arise by differentiation from unrecognizable erythroid precursor cells. Despite an apparent reduction in proliferation during the maturation of these cells, this system offers a method for investigating various aspects of erythroid cell differentiation and development.
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