Erythrocyte transketolase activity measured on the Gemstar discrete analyser

J E Buttery,C R Milner,B R Chamberlain

doi:10.1155/s1463924684000316

J E Buttery, C R Milner + Show 1 more

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https://doi.org/10.1155/s1463924684000316

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Abstract

Erythrocyte transketolase activity measured on the Gemstar discrete analyser

Highlights

The NADH-dependent method of Smeets et al [1] for erythrocyte transketolase (TK, EC 2.2.1.1) is a kinetic assay which measures the rate of decreasing absorbance ofNADH at 340 nm
A direct adaptation of Smeets’s procedure to the Gilford System 5, a discrete analyser, has been published [4]. The latter method is simple and economical, it is time-consuming for large-batch analyses, as the System 5 measures one test at a time
The procedure of Smeets et al was adapted for this machine and this paper describes the details and compares the results obtained with those derived by the manual method of Smeets on the Pye Unicam SP8000 spectrophotometer

Summary

Introduction

The NADH-dependent method of Smeets et al [1] for erythrocyte transketolase (TK, EC 2.2.1.1) is a kinetic assay which measures the rate of decreasing absorbance ofNADH at 340 nm. The TK assay has been automated using.a centrifugal analyser [-2] and the LKB Reaction Rate Analyser [3], but both techniques require deproteinization. A direct adaptation of Smeets’s procedure to the Gilford System 5, a discrete analyser, has been published [4]. The latter method is simple and economical, it is time-consuming for large-batch analyses, as the System 5 measures one test at a time. The procedure of Smeets et al was adapted for this machine and this paper describes the details and compares the results obtained with those derived by the manual method of Smeets on the Pye Unicam SP8000 spectrophotometer

Methods

Results

Conclusion