Abstract
INVESTIGATIONS to characterize the anaemia occurring in mouse lymphoid leukaemia have recently involved a comparison of the erythrocyte osmotic fragility in mice of the low-leukaemia strain C5H, and the high-leukaemia strain AKR, using the technique of Creed1. Mice were bled under ether anaesthesia by cutting the axillary blood vessels and gently aspirating the blood with a wide-bore Pasteur pipette. The blood was immediately transferred to a heparinized test-tube, and the osmotic fragility of the cells tested within 5 min of collection of the blood.
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