Abstract
This experiment investigated a blood smear method as an alternative to flow cytometry for ploidy determination in Arctic charr, Salvelinus alpinus. Fertilized eggs were either treated with hydrostatic pressure (putative triploids) or untreated (diploid controls), and the embryos, alevins, and juveniles were then reared under identical conditions. Blood samples were collected from alevins (2 g average body weight) for blood smear analysis, and from juveniles (120 g average weight) for both blood smear analysis and flow cytometry. Student's t tests revealed significant (p < 0.0001) differences in mean erythrocyte length between the two groups, with pressure‐treated fish having higher values than the control group as alevins (20.43 ± 0.53 μm and 15.90 ± 0.45 μm respectively) and juveniles (22.83 ± 0.66 μm and 17.68 ± 0.45 μm respectively). Ploidy assignment obtained with blood smear analysis perfectly matched results obtained with flow cytometry. The blood smear method is therefore a valid, simple, and less expensive alternative to flow cytometry for the assessment of ploidy level in Arctic charr.
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