Erythrina subumbrans leaves extracts improved Diabetic condition by reducing MDA and 8-OHDG on Rat model

Abstract

Erythrina subumbrans, commonly known as DadapDuri, have been used to treat diabetes by people in the West Sumatra region. This study was designed to determine E.subumbransextract on blood glucose and MDA levels in diabetic Wistar rats induced by low-dose Streptozotocin and a high-fat diet. Type II Diabetes Mellitus rats induced by High-fat diet (HFD) and followed by two times injection of a combination of Nicotinamide (110mg/kg BW) and low-dose Streptozotocin (40mg/kg BW). The rat was randomized and then divided into six groups (n=4). Diabetic rats were treated with E. subumbransextract orally in doses of 50, 100, and 200mg/200gBW once daily for three weeks. Metformin (90mg/200gBW, orally) was used as the reference drug. Blood glucose levels were measured every 7th day using a glucometer for three weeks of treatment. After treatment, the serum MDA and 8-OHdG were calculated. Intraperitoneal glucose tolerance test and intraperitoneal insulin tolerance test were performed on the last day of treatment. E. subumbransextract at a dose of 200mg/200gBW administered orally significantly (P < 0.05) could lower and normalize blood glucose levels compared to the Negative control group. The decrease in serum MDA and 8-OHdG levels during E. subumbransextract treatment at dose 3 was significantly different (P 0.05) than the HFD/STZ-NA control group. In this study, it can be concluded that E. subumbransleaves show promising hypoglycemic action and antioxidant effects starting at doses of 200mg/200gBW.