Abstract
In this study, Eryngium billardieri was collected from Patnos district of Ağrı province during the vegetation period. While some of the collected plant was stored fresh in a -86oC freezer, the other part was preserved as herbarium material. After the species of the plant were identified, methanol and pure water extracts were prepared from the aerial parts of the fresh and herbarium samples. The phenolic contents of the extracts were determined by using HPLC. Moreover, various in vitro antioxidant properties and DNA protective properties of the relevant extracts were tested. The comparison of the test results showed that the phenolic compounds of the fresh plant extracts were more than that of herbarium extracts. Although both extracts showed good antioxidant activities, their activity was lower than the standart antioxidants. Moreover, herbarium extracts were seen to have a relatively better effect on the pUC18 DNA stabilization, irrespective of the presence of H2O2 in the environment. Having considered that there is limited number of studies on E.billardieri in the literature, this study will offer important insights into the studies in this area.
Highlights
Before the discovery of synthetic drugs, almost all civilizations relied on plants to maintain their health or treat various diseases (Parham et al, 2020; Soumia, 2018)
Results of phenolic concentration The phenolic concentration of the extracts was determined from both materials using
HPLC chromatogram results of the fresh extract are shown in figure 1, and herbarium extract chromatogram results are shown in figure 2
Summary
Before the discovery of synthetic drugs, almost all civilizations relied on plants to maintain their health or treat various diseases (Parham et al, 2020; Soumia, 2018). Due to the presence of phenolics and secondary metabolites, plants have positive effects on neurodegenerative diseases such as Alzheimer’s and Parkinson’s and common problems such as phlegm, cough, sinusitis, edema, and wound healing (Nebija et al, 2009; Soumia, 2018). Apiaceae family have rich phenolic content, and the members of this family have been reported to possess positive effects on many diseases and ailments including edema, cough, bronchitis, skin and respiratory ailments, sinusitis, urinary tract infections, wound healing, infertility and inflammatory diseases (Kikowska et al, 2016; Soumia, 2018). The results obtained from these analyses were correlated with each other
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