Abstract
Journal of Gastroenterology and HepatologyVolume 37, Issue 4 p. 774-774 ErratumFree Access Erratum This article corrects the following: ePoster Volume 36Issue S2Journal of Gastroenterology and Hepatology pages: 74-283 First Published online: August 26, 2021 First published: 22 March 2022 https://doi.org/10.1111/jgh.15810AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat The abstract entitled “PP-0509 Anthocyanin Extracts from Black Rice Bran Influence the Adenosine Triphosphate-Binding Cassette Subfamily A Member 1 Gene Expression and Colorectal Cancer Cell Proliferation” by Iswanti et al.1 was missing in the published ePoster abstracts. The complete content is presented below. PP-0509 Anthocyanin Extracts from Black Rice Bran Influence the Adenosine Triphosphate-Binding Cassette Subfamily A Member 1 Gene Expression and Colorectal Cancer Cell Proliferation Authors: WIZA ISWANTI1, KARINA RAHMADIA EKAWIDYANI1,2, RINA AGUSTINA1,3, ASMARINAH4,5, SLAMET BUDIJANTO6, MURDANI ABDULLAH1,3,7,8 Affiliations: 1Department of Nutrition, Faculty of Medicine, Universitas Indonesia-Dr Cipto Mangunkusumo General Hospital, Jakarta 10430, Indonesia, 2Department of Community Nutrition, Faculty of Human Ecology, IPB University, Dramaga, Bogor 16680, Indonesia, 3Human Nutrition Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia, 4Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia, 5Molecular Biology Unit, Integrated Laboratory Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia, 6Department of Food Science and Technology, Faculty of Agricultural Technology, IPB University, Dramaga Bogor 16680, Indonesia, 7Human Cancer Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia, 8Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia-Dr. Cipto Mangunkusumo General Hospital, Jakarta 10430, Indonesia Background and Aim: Adenosine triphosphate-binding cassette subfamily A member 1 (ABCA1) gene expression is lower in colon cancer, suggesting increased cell proliferation. This study assessed the ABCA1 gene expression and colorectal cancer cell proliferation in the HT-29 cell line after given anthocyanin supplement extracted from Indonesian black rice bran. Methods: This experimental study involved three administration groups of anthocyanin extract into cell line: anthocyanin extract with a dosage of 1.6 mg/mL (D1), a dosage of 2.0 mg/mL (D2), and a control group without anthocyanin (C). The dosage was obtained after determining the half-maximal inhibitory concentration (IC50) value. Each group was triplicated and evaluated after 24 and 48 hours of cell incubation. The cell proliferation and IC50 value were defined based on 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and spectrophotometry. Gene expression was analyzed with relative quantification using quantitative polymerase chain reaction (qPCR). Results: The average proliferation proportion of HT-29 cells after 24 hours for D1 was 55.0%, and D2 was 41.4% as compared to the control group that was considered 100%. While, for the 48 hours, the proliferations were 53.1% (D1) and 25.8% (D2) as compared to 100% (C). The dosage of IC50 was set at 1.7 mg/mL. The ABCA1 gene expression in HT-29 cell after supplementation of anthocyanin extract from black rice bran with IC50 dosage and with above IC50 dosage (2.0 mg/mL) was 1.2 and 2.3 times higher than the control group, respectively. Conclusion: Increase dosage of anthocyanin supplement extracted from black rice bran may increase ABCA1 gene expression and decrease proliferation of HT-29 cell line. Further study should explore the influence of anthocyanin extract on these outcomes in more dosage variations. Keywords: anthocyanin, cell proliferation, colorectal neoplasm, functional food, mRNA Reference 1.Asia Pacific Digestive Week (APDW), Kuala Lumpur, Malaysia, 19–22 August 2021|Virtual. J. Gastroenterol. Hepatol. 2021; 36(Suppl. 2): 74– 283. https://doi.org/10.1111/jgh.15607Google Scholar Volume37, Issue4April 2022Pages 774-774 ReferencesRelatedInformation
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