Erratum

Abstract

EPPO BulletinVolume 44, Issue 1 p. 103-104 ErratumFree Access Erratum This article corrects the following: PM 7/100(1): Rep-PCR tests for identification of bacteria Volume 40Issue 3EPPO Bulletin pages: 365-368 First Published online: November 15, 2010 First published: 25 March 2014 https://doi.org/10.1111/epp.12097AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat European and Mediterranean Plant Protection Organization Organisation Européenne et Méditerranéenne pour la Protection des Plantes Diagnostics Diagnostic PM 7/100 (1) Rep-PCR tests for identification of bacteria This Standard (OEPP/EPPO, 2010) was published in December 2010 in the Diagnostic Protocol section of the EPPO Bulletin. The volumes of water included in the Mastermix tables for BOX PCR, REP PCR and ERIC PCR were incorrect. The modified figures are written in bold below. In addition the headings for the tables have been modified in line with the revised instructions for authors. The correct volumes per reaction are included below. The EPPO Bulletin would like to apologize for this error. B1) BOX-PCR Mastermix For reaction volume of 25 μL Working concentration Volume per reaction (μL) Final concentration R/DNAse-free water N/A 16.55 Reaction buffer (Invitrogen) 10 × 2.50 1 × MgCl2 (Invitrogen) 50 mM 0.75 1.5 mM dNTP mix (Promega) 10 mM each 0.50 0.2 mM BOX AIR 20 μM 2.50 2 μM PlatinumTaq (Invitrogen) 5 U μL −1 0.20 1 U DNA extract N/A 2.00 Total 25.00 B2) REP-PCR Mastermix For reaction volume of 25 μL Working concentration) Volume per reaction (μL) Final concentration R/DNAse-free water N/A 14.05 Reaction buffer (Invitrogen) 10 × 2.50 1 × MgCl2 (Invitrogen) 50 mM 0.75 1.5 mM dNTP mix (Promega) 10 mM each 0.50 0.2 mM ERIC1R 20 μM 2.50 2.0 μM ERIC2 20 μM 2.50 2.0 μM PlatinumTaq (Invitrogen) 5 U μL −1 0.20 1 U DNA extract N/A 2.00 Total 25.00 25.00 B3) ERIC-PCR Mastermix For reaction volume of 25 μL Working concentration) Volume per reaction (μL) Final concentration R/DNAse-free water N/A 14.05 Reaction buffer (Invitrogen) 10 × 2.50 1 × MgCl2 (Invitrogen) 50 mM 0.75 1.5 mM dNTP mix (Promega) 10 mM each 0.50 0.2 mM REP1R-1 20 μM 2.50 2.0 μM REP2-1 20 μM 2.50 2.0 μM PlatinumTaq (Invitrogen) 5 U μL −1 0.20 1 U DNA extract N/A 2.00 Total 25.00 25.00 Reference OEPP/EPPO (2010) EPPO Standard PM 7/100(1) Rep-PCR tests for identification of bacteria. Bulletin OEPP/EPP Bulletin 40, 365– 368. Wiley Online LibraryGoogle Scholar Volume44, Issue1April 2014Pages 103-104 ReferencesRelatedInformation