Abstract

A new aerobic, Gram-negative, chemo-organo- trophic, euryhaline bacterium, designated strain JL2009 T , was isolated from surface water of the Caribbean Sea. The strain formed flaxen colonies on marine ager 2216 (MA; Difco) medium. Cells were dimorphic, with stalks or a polar flagellum, and reproduction occurred by means of binary fission. Growth occurred at 15-45 C (optimum at 35 C), 0-15 % (w/v) NaCl (optimum at 1-10 %) and pH 5-9 (optimum at pH 7-8). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct evolutionary lineage within the family Hyphomo- nadaceae. Strain JL2009 T was most closely related to Maricaulis parjimensis MCS 25 T (92.2 % DNA sequence similarity), Woodsholea maritime CM2243 T (90.9 %), and Oceanicaulis alexandrii C116-18 T (90.9 %). The major respiratory quinone was Q-10. The dominant cellular fatty acids were summed feature 8 (C18:1 x7c), C18:0 and 11-methyl C18:1 x7c. The polar lipid pattern indicated the presence of phospholipid, phosphatidyl glycerol and gly- colipids. The G ? C content of the genomic DNA was 70.5 mol%. On the basis of the chemotaxonomic and phenotypic characteristics and the phylogenetic evidence, strain JL2009 T represents a novel genus and species in the family Hyphomonadaceae, for which the name Euryhalo- caulis caribicus gen. nov., sp. nov. is proposed. The type strain of Euryhalocaulis caribicus is JL2009 T (=CGMCC 1.12036 T = JCM 18163 T ).

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