Erratum: The locust foraging gene

Abstract

In the May 2010 article “The Locust Foraging Gene” by Lucas et al. (ARCH 2010;74:52–66), the resolution of Figures 1, 3 and 4 was poor, rendering Figure 1 unreadable. This erratum replaces those figures with higher resolution versions. Alignments of amino acid sequence of S. gregaria, L. migratoria, and D. melanogaster using Muscle (SeaView, Galtier et al., 1996). Identity and consensus sequence is shown using Geneious software. The different identified domains of the PKG are shown for D. melanogaster. FOR expression patterns in the locust brain. A: Merged confocal image of frontal 3-µm optical sections of a locust brain showing the major brain neuropils (in green, mouse antibody, nc82). B: A distinct PKG-IR region is seen as a cluster of cell bodies in the anterior midline of the brain (double staining with anti-FOR in red and nc82 in green). C: Similar FOR expression patterns can be observed in gregarious (Gre) or solitary-reared animals (Sol), in adult male (M), female (F), and larvae. Scale bars=250 µm. A: Merged confocal image of frontal 3-µm optical sections of a locust brain following neurobiotin backfilling from both left and right NCC1 nerves. The cell bodies stained are in the brain area known as the pars intercerebralis (PI) of the protocerebrum and largely overlap the area immunostained by anti-FOR (compare to Fig. 3). The area marked in A1 is enlarged in A2. Scale bars=250 µm. B: Many cell bodies in this area were yellow after double staining with anti-FOR (red) and neurobiotin backfill (green). Cells shown in B correspond to the areas marked in A2. Scale bar=10 µm.