Abstract
Human cytomegalovirus (HCMV) is a virus which has the potential to alter cellular gene expression through multiple mechanisms. With the application of DNA microarrays, we could monitor the effects of pathogens on host-cell gene expression programmes in great depth and on a broad scale. Changes in mRNA expression levels of human endothelial-like ECV304 cells following infection with human cytomegalovirus AD169 strain was analyzed by a microarray system comprising 21073 60-mer oligonucleotide probes which represent 18716 human genes or transcripts. The results from cDNA microarray showed that there were 559 differential expressed genes consisted of 471 upregulated genes and 88 down-regulated genes. Real-time qPCR was performed to validate the expression of 6 selected genes (RPS24, MGC8721, SLC27A3, MST4, TRAF2 and LRRC28), and the results of which were consistent with those from the microarray. Among 237 biology processes, 39 biology processes were found to be related significantly to HCMV-infection. The signal transduction is the most significant biological process with the lowest p value (p=0.005) among all biological process which involved in response to HCMV infection. Several of these gene products might play key roles in virus-induced pathogenesis. These findings may help to elucidate the pathogenic mechanisms of HCMV caused diseases. [This corrects the article on p. 243 in vol. 13, PMID: 24235919.].
Highlights
Human cytomegalovirus (HCMV), which is a species of the cytomegalovirus family of viruses and is alternatively known as human herpesvirus-5 (HHV5), is the most common pathogen causing congenital infection and primary infection during pregnancy[1].It can cause life-threatening systemic infections in immunocompromised patients and has been recognized as a risk factor for vascular diseases, like arterial restenosis and atherosclerosis[2]
Because most studies aimed at human foreskin fibroblast (HFF) as target cell which was infected by HCMV, gene expression profiling of endothelial cells infected HCMV has not yet been studied intensively
HCMV infection in ECV304 cells The pp[65] is used for rapid diagnosis of infected-host cells, which is expressed in the host cell nuclei during the early phase of the replication cycle (Ho et al, 1998)
Summary
Human cytomegalovirus (HCMV), which is a species of the cytomegalovirus family of viruses and is alternatively known as human herpesvirus-5 (HHV5), is the most common pathogen causing congenital infection and primary infection during pregnancy[1].It can cause life-threatening systemic infections in immunocompromised patients and has been recognized as a risk factor for vascular diseases, like arterial restenosis and atherosclerosis[2]. DNA expression profiling microarrays are designed to monitor of hundreds and thousands of mRNA levels, and analysis of those data from microarray experiment can provide us with important information to understand the biological processes and complex pathogenesis of infectious diseases This technology has been used in studies on differenet target cells infected with HCMV, which have different performance depending on probe characteristics, array density, sample labeling methods and the characteristics of target cell[11,12,13,14]. Conclusion: Several of these gene products might play key roles in virus-induced pathogenesis These findings may help to elucidate the pathogenic mechanisms of HCMV caused diseases
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