Erratum: A double missense variation of theBUB1 gene and a defective mitotic spindle checkpoint in the pancreatic cancer cell line Hs766T

Abstract

Certain samples of the cell line Hs766T within our laboratory were contaminated by the cell line RKO in the past. This affected two published papers (Hempen et al., 2002; Sohn et al., 2002, 2003). We recently identified that the reported BUB1 alteration is present in RKO cells and is not present in Hs766T cells. RKO cells are known to have an intact mitotic checkpoint (Cahill et al., 1998). Using current cell lines having identities confirmed by the sequencing of their other known mutant genes, we confirm the finding of a mitotic checkpoint defect among pancreatic cancers having CIN (chromosomal instability): the maximal mitotic index under colcemid (0.1 ug/ml) treatment is 85% for RKO cells (colorectal cancer, MSI, microsatellite instability), 9.4% for Hs766T cells (pancreatic, CIN), 23% for Su86.86 cells (pancreatic, CIN), and 44% for BxPC3 cells (pancreatic, CIN). The reported double missense mutation of the mitotic checkpoint BUB1 gene is thus associated not with a defective mitotic checkpoint in a CIN line as we had reported, but interestingly occurs in an MSI line having an intact checkpoint. The prevalence rate of BUB1 and other mitotic checkpoint gene mutations in MSI cell lines has not yet been reported. Presumably, BUB1 missense mutations do not always create a significant functional defect. © 2005 Wiley-Liss, Inc.