Errata for vol. 16, p. 355

Abstract

Erratum1 April 1997free access Over-expression of GATA-6 in Xenopus embryos blocks differentiation of heart precursors Chris Gove Chris Gove Search for more papers by this author Maggie Walmsley Maggie Walmsley Search for more papers by this author Sarbjit Nijjar Sarbjit Nijjar Search for more papers by this author David Bertwistle David Bertwistle Search for more papers by this author Matthew Guille Matthew Guille Search for more papers by this author Geoffrey Partington Geoffrey Partington Search for more papers by this author Adrian Bomford Adrian Bomford Search for more papers by this author Roger Patient Roger Patient Search for more papers by this author Chris Gove Chris Gove Search for more papers by this author Maggie Walmsley Maggie Walmsley Search for more papers by this author Sarbjit Nijjar Sarbjit Nijjar Search for more papers by this author David Bertwistle David Bertwistle Search for more papers by this author Matthew Guille Matthew Guille Search for more papers by this author Geoffrey Partington Geoffrey Partington Search for more papers by this author Adrian Bomford Adrian Bomford Search for more papers by this author Roger Patient Roger Patient Search for more papers by this author Author Information Chris Gove, Maggie Walmsley, Sarbjit Nijjar, David Bertwistle, Matthew Guille, Geoffrey Partington, Adrian Bomford and Roger Patient The EMBO Journal (1997)16:1806-1807https://doi.org/10.1093/emboj/16.7.1806 This article corrects the following: Over-expression of GATA-6 in Xenopus embryos blocks differentiation of heart precursors15 January 1997 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info In the above paper, Figures 5 and 6 were reproduced in black and white instead of in full colour. The correct versions are printed below with their legends. Figure 1.GATA-6 expression is observed in the marginal zone of gastrulae, is widely distrubuted in the ventro-lateral region in early neurula embryos and is progressively restricted to regions corresponding to the heart and blood islands in mid to late tailbud stages. Embryos were probed for GATA-6 by whole mount in situ hybridization at (A) stage 10.25, (B and B′) stage 14, (C and C′) stage 17, (D and D′) stage 20, (E and E′) stage 22, (F) stage 24, (G) stage 26, (H) stage 28 and (I) stage 35. Anterior is to the left in all views except (A) and dorsal is to the top in the lateral views. Weak staining is first detected as a ring around the vegetal pole (vp) which is more intense in the region of the dorsal lip (black arrows in A). Following gastrulation extensive ventro-lateral staining is evident in early and mid neurula stage embryos (B, B′, C and C′), by early to mid tailbud stages strong staining is evident in presumptive heart (black arrows) and blood island (white arrows) regions (D, D′, E, E′ and F). In mid to late tailbud embryos staining in the heart field and in the blood islands is progressively restricted until by stage 35 a strong signal is evident only in the heart itself (G, H and I). Download figure Download PowerPoint Figure 2.GATA-6 is expressed in the developing heart field in the antero-ventral mesoderm of mid tailbud Xenopus embryos where it co-localizes with cardiac actin, a marker of terminal cardiac differentiation. Later, as the heart tube closes, expression of GATA-6 is in the myocardium and pericardium but not the endocardium. Serial transverse sections of embryos at developmental stage 26 were hybridized in situ to digoxigenin-labelled RNA probes. (A) GATA-6 staining revealed a continuous arc of expression in the ventral mesoderm; (B) in the adjacent section probed with cardiac actin, staining was seen in the corresponding area of ventral mesoderm and unexpectedly in sensorial ectoderm; (C) the specificity of these signals is shown by the lack of staining in the adjacent section probed with the digoxigenin-labelled sense strand of GATA-6. Sections taken more posteriorly show that the ventral mesodermal expression domain of GATA-6 is increasingly separated into two symmetrical patches (D and E). The dark colour of the outer ectodermal cells (A–E) is due to pigmentation, as these are unbleached sections of non-albino embryos. (F) Viewed from the ventral aspect the staining pattern describes a V shape with the arms pointing backwards (hatched). The blood islands (stippled) and the cement gland (ellipse) are also marked. Transverse sections through whole mount embryos [position of sections indicated in (J)] hybridized in situ show: (I) GATA-6 to be expressed in the ventral mesoderm of early neurula embryos (stage 14); (G) at the closing heart tube stage (stage 28) GATA-6 is expressed in the myocardium (frequently stains blue with BM purple) and pericardium but not in the endocardium; (H) at the same stage (stage 28) transcripts for the terminal differentiation marker, XMLC2, are present in the myocardium. Download figure Download PowerPoint Next ArticlePrevious Article Volume 16Issue 71 April 1997In this issue FiguresRelatedDetailsLoading ...