Abstract

Rapidly growing mycobacteria (RGM) are common microorganisms in the environment and will lead to a variety of human infections. In recent years, case number of RGM infectious diseases has increased largely. Injection drug combined with oral macrolide is the current clinical treatment to RGM infection. However, recent studies have found that the pathogen Mycobacterium abscessus overexpresses an erythromycin resistance methyltransferase Erm(41) gene to enhance resistance against treatment with macrolide. In this study, we cloned Erm(41) gene into E. coli and the growth curve experiment demonstrated Erm(41) protein can help E. coli grow under treatment of erythromycin. We purify the Erm(41) protein for structure determination by X-ray crystallography. We also conduct drug screening to find new Erm(41) inhibitors and verify the candidate drugs with the minimum inhibitory concentration test. These results will help us to develop a new treatment to defense RGM infection.

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