Abstract
Background: Recent cytokine and chemokine research has rapidly expanded our understanding of nasal polyposis. Specific cytokines and chemokines that appear to play a central role in the pathogenesis of nasal polyposis, have now been identified. The objective of the present study was to investigate the mRNA expression of the chemokine receptor CCR3 and his ligands eotaxin and RANTES in nasal polyps in a precisely characterized patient population, with special attention to etiological factors and to compare the results with turbinate nasal mucosa of control subjects and within the patient group. Methods: Eosinophilic nasal polyps from 30 patients (comparable CT score and eosinophilia) and tissue samples taken from the inferior turbinate of 10 control subjects were obtained during endonasal surgery. Histologic examination was performed to determine the extent of eosinophilia. Realtime TaqMan PCR was performed to analyze and quantify CCR-3n eotaxin―, RANTES mRNA expression. The results were analyzed using the Mann-Whitney U test and the Spearman test. Results: In nasal polyps, CCR3― (p < 0,001) and eotaxin (p = 0,001) mRNA expression were significantly higher than in controls. There significantly correlation between CCR3― and eotaxin mRNA expression (p = 0,01). No correlation was detected between the CCR3 and RANTES mRNA values and the RANTES and eotaxin mRNA values. In nasal polyps of patients with ASS sensitivity and allergy, CCR3 mRNA expression was significantly evelated, compared with the other patients. There was no difference in RANTES mRNA expression between patients and controls, but within the patient group, non-allergic patients (p = 0,012) and allergic patients (p = 0,029) had increased RANT'ES mRNA values compared with patients suffering from ASS sensitivity and allergy. Conclusions: Our results suggest that the CCR3 eotaxin system plays a key role in the selective recruitment of eosinophils and in the development of nasal polyps. The differences in RANTES mRNA expression within the patient group indicate a disease-specific pathomechanism in patients with different etiological factors. Data may lead to the assumption that ASS sensitivity and also allergy are important etiological factors in the multifactorial genesis of polyposis nasi.
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