Abstract
An actinomycete strain, H41-59, isolated from sea sediment in a mangrove district, was identified as Streptomyces anandii on the basis of 16S rDNA gene sequence analysis as well as the investigation of its morphological, physiological and biochemical characteristics. Three new ergosterols, ananstreps A–C (1–3), along with ten known ones (4–13), were isolated from the culture broth of this strain. The gross structures of these new compounds were elucidated on the basis of extensive analysis of spectroscopic data, including HR-ESI-MS, and NMR. The cytotoxicities of these isolates against human breast adenocarcinoma cell line MCF-7, human glioblastoma cell line SF-268, and human lung cancer cell line NCI-H460 and their antibacterial activities in inhibiting the growth of Candida albicans and some other pathogenic microorganisms were tested. Compounds 3–8, 10 and 11 displayed cytotoxicity with IC50 values in a range from 13.0 to 27.8 μg/mL. However, all the tested compounds showed no activity on C. albicans and other bacteria at the test concentration of 1 mg/mL with the paper disc diffusion method.
Highlights
In recent years, many researchers have paid attention to marine-derived microorganisms because of the increasing rate of rediscovery of known compounds on terrestrial land [1]
Application of multiple chromatographic procedures and modern spectral methods led to the isolation and identification of thirteen sterols including three new sterols (1–3). Their cytotoxicities against three cancer cell lines and their antibiotic activities inhibiting the growth of C. albicans and some pathogenic bacteria were
An ethyl (EtOAc) partition from the 95% ethanol (EtOH) extract of mycelium of Streptomyces anandii strain H41-59 was subjected to repeated silica gel column chromatography and and their antibiotic activities inhibiting the growth of C. albicans and some pathogenic bacteria were purified by semi-preparative reverse phase HPLC separation to yield thirteen compounds
Summary
Many researchers have paid attention to marine-derived microorganisms because of the increasing rate of rediscovery of known compounds on terrestrial land [1]. Streptomyces are a genus of Gram-positive, filamentous bacteria usually dwelled in soil. They are one of the most diverse in species and show the ability to produce clinical useful compounds with different structures, such as streptomycins, actinomycins, adriamycin, vancomycin, and tacrolimus [8,9,10]. Application of multiple chromatographic procedures and modern spectral methods led to the isolation and identification of thirteen sterols including three new sterols (1–3). Their cytotoxicities against three cancer cell lines and their antibiotic activities inhibiting the growth of C. albicans and some pathogenic bacteria were. H41-59, and try to hypothesize the biosynthetic pathway of these sterols
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