Abstract
BackgroundMalignant melanoma (MM) is highly metastatic and has the highest mortality rate in patients with skin cancer. The ERBB3 binding protein 1 (Ebp1) has been linked to the onset and progression of a number of malignancies. However, the role of Ebp1 in MM has not yet been reported.MethodsMultiple databases were analyzed for comparing the expression of Ebp1 in normal skin and MM. Ebp1 expression was knocked down in A375 and B16 cells, and the impact of Ebp1 on the cell growth was tested by CCK-8, plate clone colony, and cell cycle assays. Scratch, transwell, and in vivo caudal vein lung metastasis tests were also used to confirm the effects of Ebp1 on melanoma cells migration, invasion, and metastasis. Furthermore, the possible molecular mechanism of Ebp1 was predicted by set enrichment analysis and verified by western blotting.ResultsEbp1 expression was substantially higher in MM than it was in normal skin, and Ebp1 was linked to the clinical stage and lymph node metastases of patients with MM. Knockdown of Ebp1 inhibited cell proliferation, migration, and invasion. In vivo experiments further verified that the knockdown of Ebp1 had an obvious inhibitory effect on lung metastasis in nude mice. Knockdown of Ebp1 reduced vimentin, N-cadherin, slug, and snail expression while increasing E-cadherin expression. Furthermore, knockdown of Ebp1 reduced the expression of β-catenin, as well as its downstream targets CyclinD1 and p-GSK3β; however, a Wnt/β-catenin agonist could reverse this effect.ConclusionEbp1 may promote the proliferation and metastasis of melanoma cells through activation of the Wnt/β-catenin pathway.Graphical
Highlights
As one of the deadliest cancers, malignant melanoma (MM) is a highly malignant tumor and difficult to treat when diagnosed in the late stage
In order to explore the mechanism of ERBB3 binding protein 1 (Ebp1) regulating Epithelial mesenchymal transition (EMT) in melanoma cells, we studied the expression of β-catenin and the downstream signaling molecules in the wnt/βcatenin pathway
Pisapia et al found that Ebp1 p48 was highly expressed in M14 melanoma cells, and Ebp1 p48 promoted the growth of glioblastoma cells and melanoma cells as an oncogene [26]
Summary
As one of the deadliest cancers, malignant melanoma (MM) is a highly malignant tumor and difficult to treat when diagnosed in the late stage. 75% of skin cancer patients die of melanoma, it accounts for approximately 2% of all skin cancers; MM is the main cause of death from skin cancer [1]. The principal treatment for early stage melanoma is surgery; 40–60% of highrisk cases experience recurrence and metastasis [3], and patients with metastatic melanoma have a five-year survival rate of only 10% [4]. In the past decades, targeted therapy has emerged as the main treatment for various cancers, and new targets have been discovered one after the other. Malignant melanoma (MM) is highly metastatic and has the highest mortality rate in patients with skin cancer. The role of Ebp in MM has not yet been reported
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
