Erasure of the paternal transcription program during spermiogenesis: The first step in the reprogramming of sperm chromatin for zygotic development

Abstract

The original article to which this Erratum refers was published in Developmental Dynamics 237:1463–1476 In the original published version of this article, several textual errors were made, along with an error in a figure. Please find the corrected text and figure provided in this Erratum, with the corrected words in boldface. The authors and publisher regret these errors. Page 1464, column 2, line 42: In this study, we examined the mechanism underlying transcription/epigenetic reprogramming and transcription silencing during spermatogenesis. Page 1468, column 3, line 27: while other CFs did not reassociate with the chromatin before pronuclear formation (Sun et al.,2007). Page 1469, column 3, line 10 from the bottom of the page: that is at a time substantially later than that when most CFs disappear from chromatin. Page 1466 and 1467, the six panels in Fig. 2 represent in sequence HDAC1, HP1α, Pol II, SRG3, TAF1, and TBP, respectively. Page 1472 and 1473, the four panels presented in this version were incorrect. The correct Fig. 5 is presented in this Erratum as follows. Reassociation of CFs to the paternal chromatin after fertilization. Note that the sperm chromatin brings into the embryo not only basic nuclear proteins, but also some residual CFs. Signals of TFIIB, Pol II, HP1α and SRG3 are shown in green and that of Hoechst, in blue. Arrows point to sperm content/male pronuclei at the marked stage. Abbreviations: <5 min, within 5 min after ICSI; <5min*, within 5 min after ICSI with chromatin decondensed before antibody staining; Ana E/L, early/late anaphase; PN1, pronuclear stage 1; PN2, pronuclear stage 2. SRG3 was not detected with both protocols within 5 min after ICSI, so only one panel is shown. Scale bar = 10 μm.