Abstract
Equine parvovirus-hepatitis (EqPV-H) was first reported in a horse that died of equine serum hepatitis in the USA in 2018, and was determined having a strong association with equine serum hepatitis in the following studies. As a newly discovered virus, the genomic sequences of only seven EqPV-H strains have been reported. Considering this, an epidemiological study was performed to investigate the prevalence of EqPV-H in equines in Guangdong Province in China, and obtain genomic sequences of the field prevalent EqPV-H strains. The detection rate of EqPV-H was finally determined to be 8.33% (95% CI: 2.8–18.4%), and EqPV-H's coinfection with equine hepacivirus and equine pegivirus was also determined. Then, the genomes of the Chinese field EqPV-H strains were obtained by PCR, sequencing, and assembly. Through bootscanning analysis, Simplot analysis, and phylogenetic analysis, strong evidence for natural recombination events were found in two Chinese field EqPV-H strains. The natural recombination events occurred between the Chinese and American strains, and were determined within VP protein. Finally, the genetic distance of EqPV-H strains was investigated. Nucleotide identities of 97.1–99.9% and 95.2–100% were found for NS and VP between EqPV-H strains, respectively. Together with other molecular evidence obtained in the present study, the genetic diversity of EqPV-H was determined. Taken together, the results of the present study expand our knowledge on the epidemiological characteristics, genetic variability, and evolution of EqPV-H.
Highlights
Equine serum hepatitis is considered to be one of the most common causes of acute hepatitis and liver failure in horses
Theiler’s disease-associated virus (TDAV) RNA was detected in affected horses and in administered antitoxin samples; TDAV was considered as the possible agent of equine serum hepatitis
equine parvovirus-hepatitis (EqPV-H) was first discovered in a horse that died of equine serum hepatitis in the USA in 2018 [3]
Summary
Equine serum hepatitis ( known as Theiler’s disease, acute hepatic failure, acute serum hepatitis, idiopathic acute hepatic disease, or postvaccinal hepatitis) is considered to be one of the most common causes of acute hepatitis and liver failure in horses This disease was first described in 1919 by Arnold Theiler and has often been associated with administration of equine blood products [1]. TDAV RNA was detected in affected horses and in administered antitoxin samples; TDAV was considered as the possible agent of equine serum hepatitis. This virus has only been observed in Brazilian equines since its first identification [4]. No TDAV RNA was detected in any of the cases These preliminary data demonstrated the causative association between EqPV-H, rather than TDAV, and equine serum hepatitis
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
