Abstract

The present study describes the coordination properties of a reduced Schiff base, N-(2-hydroxybenzyl)alanine, towards cobalt(II) using potentiometric as well as spectroscopic (UV-Vis and ESI-MS) methods. The results indicate the formation of six mononuclear complexes showing high stability in aqueous solution. Coordination occurs in the {O−phenolic,N,O−carboxyl} and {N,O−carboxyl} chelation modes, depending on the degree of ligand deprotonation. Examination of the complexation equilibria at pH ca 7, which is important from a biological point of view, allowed to identify two species: [CoL] and [CoL2H]−. The kinetic analysis showed a structural change of those cobalt(II) complexes from octahedral to tetrahedral in accordance with a first-order time relationship. The antimicrobial properties of N-(2-hydroxybenzyl)alanine, cobalt(II) nitrate and of the Co(II) – ligand complexes were determined against Gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis), Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli, Helicobacter pylori) and a fungal strain (Candida). The results indicate that the complexes are more active for more strains than the ligand alone. Nevertheless, the complexes induce a higher decrease in the metabolic activity of cells but without damage to nuclei. Tetrahedral structures show stronger anti-cellular toxicity than octahedral complexes, which is most likely due to the higher accessibility of the cobalt(II) center.

Highlights

  • Schiff bases, or their reduced forms, can stabilize various transition metal complexes due to their strong chelating ability towards metal ions [1,2,3]

  • Standard deviations in parentheses after overall protonation and stability constants refer to random errors only

  • Cobalt(II) complexes with a protonated phenolic group in the ligand molecule were formed in an aqueous solution

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Summary

Introduction

Their reduced forms, can stabilize various transition metal complexes due to their strong chelating ability towards metal ions [1,2,3]. Schiff bases, or their reduced forms, can stabilize various transition metal complexes due to 2their strong chelating ability towards metal ions [1,2,3]. Amino acids play important roles in various biochemical processes as endogenous ligands; in addition, acids play important roles in various biochemical processes as endogenous ligands; in addition,their their complexes complexes with with biologically-active biologically-active metal metal ions ions have have proved proved to to be be useful useful antibacterial antibacterial agents agents and and metalloprotein models [7,8,9]

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