Abstract
Equilibria between the membranes of erythrocytes as well as thrombocytes and solution ions in fatal accidental hypothermia were analyzed using a theoretical four-equilibria model. The model was developed to determinate parameters characterizing cell membrane—surrounding ion interactions: the total surface concentrations of both acidic and basic groups CA, CB, and association constants KAH, KBOH. Knowledge of these parameters was necessary to calculate the theoretical values of surface charge density. The model was validated by curve-fitting the experimental data points to simulated data generated by the model. The experimental and theoretical surface charge density values agree at pH 2–8, at higher pH the deviation was observed.
Highlights
Biological membranes are complex heterogeneous nanostructures containing a variety of compounds
Due to the lack of published studies concerning the effect of hypothermia on equilibria between cell membranes and surrounding ions, in this paper, we provide a quantitative description of the equilibria
Calculated concentration of membrane acidic (cA), cB, KAH; and KBOH values were substituted into Eq 3 to obtain surface charge densities’ theoretical data for erythrocytes and thrombocytes membranes in fatal accidental hypothermia
Summary
Biological membranes are complex heterogeneous nanostructures containing a variety of compounds. Abstract Equilibria between the membranes of erythrocytes as well as thrombocytes and solution ions in fatal accidental hypothermia were analyzed using a theoretical fourequilibria model. Keywords Fatal accidental hypothermia Á Surface charge density Á Erythrocytes Á Thrombocytes Á Microelectrophoresis Á Acid–base equilibria
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