Equilibration between cytochrome f and P700 in intact leaves

Abstract

Electron transport between the two photosynthetic reaction centres of high plants is mediated by plastoquinone, a rieske iron–sulfur centre, cytochrome f and plastocyanin. Measurements of redox equilibration amongst these have produced confusing results, with apparent equilibrium constants being estimated that are inconsistent with in vitro measurements of redox midpoint potentials of the components concerned. We have critically reexamined methods for deconvoluting cytochrome f absorbance signals in intact leaves. We have determined the decay of cytochrome f + following light to dark transitions from steady state and compared this with the decay of the oxidised photosystem I primary donor, P700 +. Measurements across a wide range of different irradiances and CO 2 concentrations were all consistent with cyt f and P700 existing in redox equilibrium, with a potential difference of around 117 mV. These results are discussed in relation to our understanding of the organisation of the photosynthetic electron transport. They also have implications for measurements of PSI electron flux—provided more than about 20% of P700 + is oxidised in the light, then the initial decay in the concentration of P700 + following a light to dark transition provides a good estimate of electron flux through PSI. Where P700 is largely reduced in the light, net reduction of cyt f + might need to be corrected for.