Abstract

Microorganisms capable of degrading the herbicide EPTC (S-ethyl N,N-dipropylcarbamothioate) were isolated from three soils with and without histories of carbamothioate use. All EPTC-degrading isolates belonged to the genus Rhodococcus. All three isolates in pure culture systems degraded 50 micrograms mL-1 of technical EPTC in as little as 14 h at low cell densities and used the molecule as a sole source of carbon and energy. Growth of the isolates in rich media led to the frequent loss of EPTC-degrading ability, although plasmids encoding for EPTC degradation could not be identified. [14C]-EPTC experiments suggested that the degradation of EPTC proceeds by initial attack at the carbonyl linkage, followed by degradation of the dipropylamine side chain.

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