Abstract
Two different nucleic acid hybridization techniques were used to search for Epstein-Barr virus (EBV) DNA in four human lymphoma-derived cell lines of B lymphocyte type: BJAB, Ramos, U-698 M, and U-715 M. These cell lines do not express an EBV-associated nuclear antigen, EBNA. Four stably EBNA-positive sublines, GC-BJAB, AW-Ramos, EHRA-Ramos, and EHRB-Ramos, obtained by exposure of the parent lines BJAB and Ramos to EBV in vitro, were also investigated. The EBNA-negative cell lines did not have detectable amounts of EBV DNA (less than 0.3 EBV genome per cell), while the EBNA-positive sublines contained EBV DNA sequences. However, two of the latter four lines had much less EBV DNA (0.8–2 viral genome equivalents per cell) than typical human lymphoid cell lines.
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