口腔癌與Epstein - Barr Virus相關性之研究

Abstract

Tobacco and alcohol are well-known causative factors of oral cancer. However, people may get this cancer independent of above stimulants. Some biological factors, such as viruses, which have been found in association with oral cancer. Epstein-Barr virus (EBV) is linked etiologically to Burkitt’s lymphoma, nasopharyngeal carcinoma, and a number of visceral carcinomas. Moreover, oral cancer had been related to various EBV. We made the gene chip with EBV genomic DNA, to confirm the EBV-chip was well down. Utilizing the total RNA extracted derived from EBV infected cell line and performed the hybridization and color development. Afterward, we used the EBV-chip to examine the Epstein-Barr virus from oral cancer patient’s tissues. We took oral cancer patient’s tissues to spy the Epstein-Barr virus by the EBV-chip and select the associated EBV expression genes. We found 82 percent of the results of EBV-chip pattern exhibited the EBV lytic cycle. Consequently, we picked out two EBV expression genes BCRF-1 and BMRF-2 from EBV-chip by hybridization. Immunohistochemistry stain of oral cancer tissues demonstrated the EBV didn’t exist. Furthermore, we used in situ hybridization with Epstein-Barr virus encoded small nuclear RNAs (EBER), unfortunately the outcomes were negative. We had already constructed these two genes with different plasmid vectors. We’ll investigate the relationship between Epstein-Barr virus and oral cancer and associated with tumorgenesis in the further.