Epsc Involved in the Encoding of Exopolysaccharides Produced by Bacillus amyloliquefaciens FZB42 Act to Boost the Drought Tolerance of Arabidopsis thaliana.

Ruo-Yu Wang,Liang Yue,Zhong-Kui Xie,Yu-Bao Zhang,Xia Zhao,Shao-Fang Liu,Xiang Lu

doi:10.3390/ijms19123795

Abstract

Bacillus amyloliquefaciens FZB42 is a plant growth-promoting rhizobacteria that stimulates plant growth, and enhances resistance to pathogens and tolerance of salt stress. Instead, the mechanistic basis of drought tolerance in Arabidopsis thaliana induced by FZB42 remains unexplored. Here, we constructed an exopolysaccharide-deficient mutant epsC and determined the role of epsC in FZB42-induced drought tolerance in A. thaliana. Results showed that FZB42 significantly enhanced growth and drought tolerance of Arabidopsis by increasing the survival rate, fresh and dry shoot weights, primary root length, root dry weight, lateral root number, and total lateral root length. Coordinated changes were also observed in cellular defense responses, including elevated concentrations of proline and activities of superoxide dismutase and peroxidase, decreased concentrations of malondialdehyde, and accumulation of hydrogen peroxide in plants treated with FZB42. The relative expression levels of drought defense-related marker genes, such as RD29A, RD17, ERD1, and LEA14, were also increased in the leaves of FZB42-treated plants. In addition, FZB42 induced the drought tolerance in Arabidopsis by the action of both ethylene and jasmonate, but not abscisic acid. However, plants inoculated with mutant strain epsC were less able to resist drought stress with respect to each of these parameters, indicating that epsC are required for the full benefit of FZB42 inoculation to be gained. Moreover, the mutant strain was less capable of supporting the formation of a biofilm and of colonizing the A. thaliana root. Therefore, epsC is an important factor that allows FZB42 to colonize the roots and induce systemic drought tolerance in Arabidopsis.

Highlights

Tissue dehydration imposed by drought can cause irreversible cellular damage, with a consequential loss in the economic yield of crop plants [1]
Other studies have determined that their presence regulates a number of phytohormones, such as abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), and ethylene (ET), thereby influencing many plant signaling networks, including those involved in the abiotic stress response [14,15,16,17]
We found that FZB42 could induce drought tolerance in Arabidopsis via ethylene and jasmonate-mediated pathways and the epsC mutant had a decreased capacity for inducing drought tolerance

Summary

Introduction

Tissue dehydration imposed by drought can cause irreversible cellular damage, with a consequential loss in the economic yield of crop plants [1]. B. amyloliquefaciens FZB42 collagen-like proteins encoding genes, including clpA, clpB, clpC, and clpD, have been reported to be crucial for biofilm formation and adhesion to plant roots [26]. Many microbes, especially those belonging to certain bacterial and fungal species, secrete exopolysaccharides, large carbohydrate polymers formed by the concatenation of monosaccharides through glycosidic bonds [27]. Especially those belonging to certain bacterial and fungal species, secrete exopolysaccharides, large carbohydrate polymers formed by the concatenation of monosaccharides through glycosidic bonds [27] These compounds have been shown to be protective of plants challenged by abiotic stress [28,29]. We suggest that exopolysaccharides encoding gene epsC plays a crucial role in allowing FZB42 to improve the tolerance of drought by Arabidopsis

Results

Analysis of the Monosaccharide Composition FZB42 Exopolysaccharide

Biofilm Formation and Growth of FZB42 and the epsC Mutant

Stomatal Aperture Measurement

Physiological Assays