EPR Spectroscopic Examination of Different Types of Paramagnetic Centers in the Blood in the Course of Burn Healing.

Katarzyna Komosinska-Vassev,Ryszard Krzyminiewski,Jerzy Stojko,Paweł Olczyk,Diana Ivanova,Krystyna Olczyk,Bernadeta Dobosz,Barbara Pilawa,Paweł Ramos,Mateusz Stojko,Janusz Kasperczyk

doi:10.1155/2019/7506274

Abstract

The multicomponent electron paramagnetic resonance spectra of the blood during healing of skin burned wounds treated with a new generation biodegradable dressings containing poly(lactide-co-glycolide) were analysed. The evolution of different types of paramagnetic centers in the blood with time of healing was determined. The EPR spectra of the blood samples at 230 K temperature were measured at 1, 10, and 21 days after burning of the pig skin. The EPR lines of the following paramagnetic centers: the high-spin Fe3+ in methemoglobin (line I), high-spin Fe3+ in transferrin (line II), and Cu2+ in ceruloplasmin and free radicals (line III) were observed in the X-band (9.3 GHz) spectra of the blood. The multicomponent structure of the EPR spectra of the tested blood samples depended on the time of the healing of the burned wounds. The amount of the high-spin Fe3+ in methemoglobin (line I) in the blood decreased after 21 days of the healing of the burned wounds. The amount of the high-spin Fe3+ in transferrin (line II) slightly increased after 21 days of therapy with the basis. The amount of Cu2+ in ceruloplasmin and free radicals (line III) in the blood was very high after 10 days of therapy. At the first day of the healing of the burned wounds, the highest amount of the high-spin Fe3+ in methemoglobin (line I), the relatively lower amounts of the high-spin Fe3+ in transferrin (line II), and Cu2+ in ceruloplasmin and free radicals (line III) existed in the blood. In the medium phase (after 10 days) of the healing of the burned wounds, the extremely higher amounts of Cu2+ in ceruloplasmin and free radicals (line III) appeared in the blood. In the last phase (after 21 days), only the low differences between the amounts of the high-spin Fe3+ in methemoglobin (line I), the high-spin Fe3+ in transferrin (line II), and Cu2+ in ceruloplasmin and free radicals (line III) were observed. The present study may serve as a starting point for the development of a new technique for monitoring molecular complexes containing iron Fe3+ (methemoglobin, transferrin) or copper Cu2+ ions (ceruloplasmin) and free radicals in the blood during wound healing.

Highlights

Thermal burns represent one of the most challenging types of trauma
A significant increase in electron paramagnetic resonance (EPR) signal from Cu2+ ions in ceruloplasmin and from free radicals (g = 2 05) recorded in the blood on the 10th day of the burn healing process was found in our study, indicating that copper and free radical are essential participants in the late phase of inflammation, and the early phase of proliferation and remodeling, which occur at this time
The X-band (9.3 GHz) electron paramagnetic resonance (EPR) examination of the paramagnetic centers in the blood obtained from the pig during healing the burned wounds dressed with a new generation biodegradable dressings containing poly(lactide-co-glycolide) indicated the following: (1) The multicomponent EPR spectra as the superposition of the lines of the high-spin Fe3+ in methemoglobin, high-spin Fe3+ in transferrin, and Cu2+ in ceruloplasmin and free radicals were characteristic for the analysed blood samples and were dependent on the time of the healing of the burned wounds

Summary

Introduction

Thermal burns represent one of the most challenging types of trauma. Injuries range from mild reddening of the skin to severe tissue damage with additional systemic complications. Mainly from activated neutrophils, are contributory to local tissue damage following thermal injury [4, 5]. Except for the increased formation of free radicals, the local and systemic inflammatory response to thermal injury involves the increased hepatic synthesis of a number of plasma proteins, which can markedly facilitate wound healing. Through its promotion of vascular endothelial growth factor, copper may accelerate wound healing by stimulating angiogenesis [16, 17] Another EPR investigations of paramagnetic centers in the whole blood during the healing process after burn traumatic injury included the molecular complexes containing Fe3+ such as transferrin and methemoglobin.

Experimental

Results and Discussion

Conclusions