Abstract

THE use of water as the electron donor and the consequent evolution of oxygen is one of the most important properties of photosynthesis by higher plants and algae. The properties of the enzyme systems involved in water oxidation are essentially unknown, however. Two new electron paramagnetic resonance (EPR) signals observed at low temperature in oxygen evolving chloroplasts are described here. The signals disappear and appear in response to short flash illumination, before freezing, of the chloroplasts, presumably due to changes in oxidation state of the components giving rise to the signals. The changes occur in response to a cycle of four flashes; it is suggested that they may reflect the oxidation states of the oxygen evolving system of the chloroplasts.

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