EPR characterization of vanadyl(IV) species in blood cells of ascidians

Abstract

EPR spectra due to vanadyl species of blood cells of Ascidia ahodori collected from different places in Japan (Ushimado and Asamushi) were measured. The EPR spectral pattern as well as their EPR parameters for these two species were found to be different from each other. EPR analysis correlating g and A indicated that vanadyl ion in the animals from Ushimado was in a much stronger ligand field than those from Asamushi. Vanadyl speices in the Ushimado animals was comparable to the stable complexes such as the vanadyl-ATP complex prepared at pH 12.