Epothilone B loaded in acellular nerve allograft enhanced sciatic nerve regeneration in rats.

Abstract

Epothilone B (EpoB) is a microtubule-stabilizing agent with neuroprotective properties. This study examines the regenerative properties of ANA supplemented with EpoB on a sciatic nerve deficit in male Wistar rats. For this purpose, the 10mm nerve gap was filled with acellular nerve allografts (ANAs) containing EpoB at 0.1, 1, and 10nM concentrations. The sensorimotor recovery was evaluated up to 16 weeks after the operation. Real-time PCR, histomorphometry analysis, and electrophysiological evaluation were also used to evaluate the process of nerve regeneration. ANA/EpoB (0.1nM) significantly improved sensorimotor recovery in rats compared to ANA, ANA/EpoB (1nM), and ANA/EpoB (10nM) groups. This led to reduced muscle atrophy, improved sciatic functional index, and thermal paw withdrawal reflex latency, indicating nerve regeneration and target organ reinnervation. The electrophysiological and histomorphometry findings also confirmed the ANA/EpoB regenerative properties (0.1nM). EpoB only enhanced ANA regenerative properties at 0.1nM, with no therapeutic effects at higher concentrations. Totally, we concluded that ANA loaded with 0.1nM EpoB can effectively reconstruct the transected sciatic nerve in rats, likely by enhancing axonal sprouting and extension.