Epitope analysis of infectious pancreatic necrosis virus (IPNV) isolated from lake trout, Salvelinus namaycush (Walbaum), by monoclonal antibodies

Abstract

Abstract. A panel of 15 monoclonal antibodies (MAbs) were raised against infeetious panercretic mecrosis virus (IPNV) associated with lake trout.salvelinus namaycush (Walwaum). (LT‐IPNV) in Cornwall Lake Alberta, for LT‐IPNV epietope analysis and comparison with other Canadian IPNV isolates. All the MAbs reacted with IPNV VP2 polypeptide in western blot and 10 MAbs were neutralizing. Both conformation and sequence dependent epitopes were found to be present on the IPNV VP, protein. The antibodies reeognized different epitopes on VP, protein in reeiproeal bloeking ELISA. Twelve MAbs reeognized common epitopes present on LT‐IPNV and IPNV from Aretic char. Salvelinus alpinus (L.), (AC‐IPNV) in binding and neutralization assays. Three MAbs reacted only with LT‐IPNV indicating that it has distinct epitopes, and thus clerly differentiaing it from AC‐IPNV isolated from the adjacent Northwest Trritories.Only two MAbs bound to Ja and BCI‐IPNV isolate and none of the MAbs neutralized these two IPNV isolates. LT‐IPNV was found to be distinct isolate, more colosly related to AC‐IPNV and Canda ‐2 than to Ja‐IPNV from alberta or other isolates in Canda. Additionally, the panel of MAbs could differnciate all the propsed Canadian IPNV scrotypes, namely C1. C2. C3 and Ja.