Abstract
Five human human-mouse hybridomas secreting human monoclonal antibodies to rice allergens were established. Antibodies secreted from the hybridomas reacted with 14–16 kDa rice major allergen proteins. Analysis with overlapping peptides synthesized on a multi-pin apparatus revealed a binding sequence of the major rice allergen protein RA17. The antigenic determinant was located in the C-terminus region of the RA17 protein.
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