Epithelial cells in the oviduct and vagina and steroid-synthesizing cells in the rabbit ovary express AhR and ARNT.

Abstract

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor. Beside exogenous ligands, an increasing list of endogenous ligands has recently been described. The AhR and its dimerization partner AhR nuclear translocator (ARNT) were found in embryos, fetuses and in genital tract tissue. Its role in reproduction and fertility is not known. In the current study, AhR and ARNT expression and co-localization were studied immunohistochemicaly during the pre-implantation period in various genital tract organs (ovary, oviduct, vagina) of the rabbit. In the ovary, the AhR was expressed in the steroid-secreting interstitial cells, in follicular and granulosa cells, and in lutein cells. The receptor was localized in the cytoplasm. A cytoplasmic localization was also found in the oviduct epithelium with a diffuse cytoplasmic staining in the ampulla and a localized cytoplasmic localization in the isthmus. In the vagina, AhR localization changed from cytoplasmic in the non-pregnant animal to nuclear staining in the basal layer of the vaginal epithelium on day 6 of pregnancy. The ARNT protein was found in all AhR expressing cells except for oocytes within primordial follicles. Its localization was in the cytoplasm and/or nuclei. Therefore, the full complement for AhR/ARNT transcriptional activity was found in the studied organs. AhR expression showed stage-specific changes in both the uterus (Hasan and Fischer 2001) and the vagina during the pre-implantation period, while the ARNT protein exhibited no change in expression during this period. In summary, these findings indicate a functional AhR/ARNT complex in the rabbit genital tract epithelia. Its precise role and activation mechanism in reproductive tissue is not clear. It is supposed, however, that this complex may be involved in both hormone production and hormone-induced cellular changes during early pregnancy of the rabbit.