Epistasis together with partial dominance, over-dominance and QTL by environment interactions contribute to yield heterosis in upland cotton.

Abstract

QTL mapping based on backcross and RIL populations suggests that epistasis together with partial dominance, over-dominance and their environmental interactions of QTLs play an important role in yield heterosis in upland cotton. A backcross population (BC) was constructed to explore the genetic basis of heterosis in upland cotton (Gossypium hirsutum L.). For yield and yield components, recombinant inbred line (RIL) and BC populations were evaluated simultaneously at three different locations. A total of 35 and 30 quantitative trait loci (QTLs) were detected based on the RILs and BC data, respectively. Six (16.7%) additive QTLs, 19 (52.8%) partial dominant QTLs and 11 (30.6%) over-dominant QTLs were detected by single-locus analysis using composite interval mapping in BC population. QTLs detected for mid-parent heterosis (MPH) were mostly related to those detected in the BC population. No significant correlation was found between marker heterozygosity and performance. It indicated that heterozygosity was not always favorable for performance. Two-locus analysis revealed 46, 25 and 12 QTLs with main effects (M-QTLs), and 55, 63 and 33 QTLs involved in digenic interactions (E-QTLs) were detected for yield and yield components in RIL, BC and MPH, respectively. A large number of M-QTLs and E-QTLs showed QTL by environment interactions (QEs) in three environments. These results suggest that epistasis together with partial dominance, over-dominance and QEs all contribute to yield heterosis in upland cotton.