Epiphytic foraminifers as indicators of heavy-metal pollution in Posidonia oceanica seagrass meadows

Abstract

Because of their toxicity, persistence and difficult biodegradability heavy metals are one of the most significant pollutants in marine environments, including seagrass meadows. Epiphytic foraminifers are conspicuous in the Posidonia oceanica meadows and can be utilized as cost-effective bioindicators. To evaluate the ecological conditions of P. oceanica meadows around the Balearic Islands four indices based on benthic foraminiferal assemblages, such as, the modified FORAM Index (FI’), the “Long vs Short life span” index (ILS), the Foram Stress Index (FSI), and Shannon-Weaver index (H’), were calculated. High index values for all sampling sites with different anthropogenic activities indicated a good ecological status of the seagrass. In contrast, the proportion of abnormal foraminiferal tests (FAI), based on morphological analysis, was variable among the study sites and reach very high abundances in areas with a priori low anthropogenic impact. Although there is not a univocal cause-effect pattern between the occurrence of deformed individuals and heavy metal pollution (such as Cu, Zn, Cd, Pb, Co, Ni, As and Sn), abnormal growth forms were significantly more abundant in sites where the tests contained higher concentrations of trace elements, and certain deformities (occurrence of protuberances and supernumerary chambers) seemed to be associated with specific pollutants (Zn, Ni and As). The disparity between the foraminiferal biotic indices and the percentage of aberrant forms associated with the heavy metal uptake can be explained by differences in the type of environmental impact and the mineral composition of the foraminiferal tests. Thus, the use of foraminifera as bioindicators, combining different approaches such as ecological indices, quantification of abnormal growth patterns and geochemical analysis of their tests, are very helpful in determining the health of seagrass meadows ecosystems. The indices are proxies to show dominant conditions over a large area, whereas the morphological and geochemical analysis of the foraminiferal tests shows very localized but long-lasting impacts with sublethal effects.