Epiphytic bacteria antagonistic to Curvularia leaf spot of yam

Abstract

Curvularia eragrostidis yam leaf spot is a serious concern among the northeast Brazilian yam growing areas. In order to study its biocontrol, bacterial isolates from the yam phylloplane were tested against the pathogen. They were evaluated with respect to the following parameters: (1) inhibition of C. eragrostidis mycelial growth by using paired culture and cellophane membrane methods, (2) inhibition of conidium germination by using a paired suspension test, (3) reduction of disease severity and, (4) persistence of antagonistic action, on plants under greenhouse conditions. From a total of 162 bacterial isolates, 39 showed antagonism to the pathogen in paired culture. The bacteria produced extracellular, nonvolatile, and diffusible metabolites in the membrane cellophane test. Seventeen isolates resulted in more than 75% inhibition of C. eragrostidis mycelial growth. Among them, IF-26 showed the greatest antagonism. The isolates IF-82, IF-88, and IF-109 inhibited pathogen conidial germination, with average inhibition levels of 99.2, 98.2 and 96.2%, respectively. Under greenhouse conditions the antagonists were applied at three different time intervals relative to C. eragrostidis inoculation: 3 days before, at the same time, and 3 days after. IF-82 and IF-88 applied at the same time as pathogen inoculation both reduced disease severity 75%. IF-82 showed the best persistence of antagonistic action, with an average of 96.3%. IF-82, identified as Bacillus subtilis, was the best biocontrol agent for the yam leaf spot disease in this study.