Abstract
A sugar, previously unobserved, was formed from cellobiose by enzymes in the supernatant fraction of Ruminococcus albus cultures. The sugar was isolated by preparative paper chromatography and was subsequently identified by means of its melting point, optical rotation, infrared spectrum, and chromatographie behavior as 4- O-β- d-glucopyranosyl- d-mannose. The epimerase was assayed by determining the conversion of cellobiose into glucosylmannose by means of quantitative paper chromatography. Maximum activity occurs near pH 7.0. The enzyme is unstable at room temperature and loses activity rapidly above 38 °. At equilibrium, the enzymic interconversion favors the cellobiose configuration. The reversible epimerization of cellobiose and glucosylmannose apparently occurs through a direct reaction at carbon-2 of the reducing moiety of the disaccharide.
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